降脂灵片中决明子定性和定量分析方法的研究

The study on qualitative analysis and quantitative analysis method of Semen Cassiae in JiangZhiLing tablet
http://scholar.ilib.cn/A-tianjykdxxb200101014.html
<<天津医科大学学报 >>2001年01期ZHANG Qi

[摘要]目的：通过对决明子的定性和定量分析来控制降脂灵片的质量。方法：采用薄层层析法对决明子进行定性分析；应用高效液相色谱法，采用ODS-C18色谱柱，以甲醇：0.1％高氯酸(80:20)为流动相，检测波长为365nm，测定决明子中大黄素的含量。结果：在0.05～0.8mg/L浓度范围内，线性关系良好，回归方程为Y=32.92+5391.86X，r=0.9998，平均回收率为98.70%。结论：本法简便可靠准确，可以作为质控方法。Objective :The quality of JiangZhiLing tablet was controlled based on the qualitative analysis and quantitative analysis of Semen Cassiae. Methods: Qualitative analysis of Semen Cassiae was performed by TLC (thin-layer-chromatograph); HPLC(high-performance liquid chromatograph) was applied to determine the quantity of Emodin in Semen Cassiae at 365nm wavelength, in which ODS-C18 volume, 0.1%methanol flow phase and PCA (perchloric acid)were used. Results: Emodin' s detection linear range is 0.05～0.8mg/L. The regression equation is Y=33.92+5391.86X, r=0.9998, average recovery is 98.70%. Conclusion: This method is simple and reliable enough to be used as the method of quality-control. 关键词： 降脂灵片 , 决明子 , 大黄素 , 薄层层析法 , 高效液相色谱法